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anti igg antibodies  (Proteintech)


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    Structured Review

    Proteintech anti igg antibodies
    Anti Igg Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+fus+antibody/FUS%2FTLS+Antibody/10__1016_slash_j__jrras__2026__102285-82-15-21
    Average 94 stars, based on 130 article reviews
    anti igg antibodies - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Role of thrombus-derived exosomal lncRNA LOC101928697 in regulating endothelial function via FUS protein interaction in myocardial infarction.
    Article Snippet: Human cell lysates were prepared using IP lysis buffer (Beyotime, Cat# P0013), precleared, and incubated with the RNA-coated beads at 4°C for 2–4 h with rotation. .. After extensive washing, the bound proteins were eluted, separated by SDS-PAGE, and analyzed by Western blot using anti-FUS antibody (Proteintech, Cat No. 11570-1-AP, dilution 1:5000) to detect the enrichment of FUS protein. ..

    Article Title: Role of thrombus-derived exosomal lncRNA LOC101928697 in regulating endothelial function via FUS protein interaction in myocardial infarction
    Article Snippet: Human cell lysates were prepared using IP lysis buffer (Beyotime, Cat# P0013), pre-cleared, and incubated with the RNA-coated beads at 4°C for 2–4 h with rotation. .. After extensive washing, the bound proteins were eluted, separated by SDS-PAGE, and analyzed by Western blot using anti-FUS antibody (Proteintech, Cat No. 11570-1-AP, dilution 1:5000) to detect the enrichment of FUS protein. ..

    Incubation:

    Article Title: FUS post-transcriptional splicing is autoregulated via RNA condensation with therapeutic potential for ALS-FUS
    Article Snippet: .. The coverslips were blocked by 1% BSA in PBS for 1 h at RT and subsequently incubated with an anti-FUS antibody (1:5000, rabbit polyclonal, Proteintech 11570-1-AP) for 2 h at RT. .. After PBS washes, an anti-rabbit AlexaFluor546 antibody (1:1000, Invitrogen) was applied for 1 h. The coverslips were mounted with Immu-mount (ThermoFisher) and imaged using Olympus BX57 upright microscope and ORCA-Flash 4.0 camera and processed using cellSens Dimension software (Olympus).

    Article Title: M6A-dependent RNA condensation underlies FUS autoregulation and can be harnessed for ALS therapy development
    Article Snippet: .. The coverslips were blocked in 1% BSA in PBS for 1 hour at RT and subsequently incubated with an anti-FUS antibody (1:5000; rabbit polyclonal, Proteintech, 11570-1-AP, RRID:AB_2247082) for 2 hours at RT. .. After PBS washes, an anti-rabbit Alexa Fluor 546 antibody (1:1000; Invitrogen) was applied for 1 hour.

    Article Title: circLRCH3-FUS interaction stabilizes IFI35 mRNA, inhibiting autophagy and aggravating podocyte pyroptosis in lupus nephritis.
    Article Snippet: Background: Lupus nephritis (LN) can severely compromise kidney function, with podocyte injury recognized as a key pathogenic event.. This study aimed to elucidate the role of circular RNA circLRCH3 in LN and to clarify its underlying molecular mechanisms.. Methods: The expression of circLRCH3 was evaluated in renal tissues and serum samples from LN patients, as well as in human podocyte cells (HPCs) treated with LN-derived immunoglobulin G (LN-IgG).

    Article Title: Splicing factor FUS facilitates the progression of PIT1-lineage PitNETs by upregulating MDM2
    Article Snippet: GH3 and MMQ cells were lysed and subjected to RIP with an EZ-Magna kit (17-701, Merck Millipore). .. After overnight incubation at 4 °C with magnetic beads coated with anti-FUS antibody (11570-1-AP, Proteintech), the immunocomplexes were washed 6 times and digested with proteinase K. Total RNA was isolated with phenol/chloroform/isoamyl alcohol (125:24:1), followed by qPCR or sequencing (LC Bio Corporation). ..

    Saline:

    Article Title: Pathologic changes in neuronal intranuclear inclusion disease are linked to aberrant FUS interaction under hyperosmotic stress.
    Article Snippet: Selected sections were immunostained with primary antibodies against N2CpolyG (4D12) (Boivin et al., 2021) and p62 (1:200, Mouse, Abcam, ab56416). .. Cells were rinsed with 1× phosphatebuffered saline (PBS), fixed with 4% polyformaldehyde in 1× PBS, and stained with anti-FUS antibody (1:200, Rabbit, Proteintech, # 11570–1-AP), and anti-TIA1 antibody (1:100, Rabbit, Proteintech, # 12133–2-AP) at 48-h post-transduction with corresponding adenovirus. ..

    Staining:

    Article Title: Pathologic changes in neuronal intranuclear inclusion disease are linked to aberrant FUS interaction under hyperosmotic stress.
    Article Snippet: Selected sections were immunostained with primary antibodies against N2CpolyG (4D12) (Boivin et al., 2021) and p62 (1:200, Mouse, Abcam, ab56416). .. Cells were rinsed with 1× phosphatebuffered saline (PBS), fixed with 4% polyformaldehyde in 1× PBS, and stained with anti-FUS antibody (1:200, Rabbit, Proteintech, # 11570–1-AP), and anti-TIA1 antibody (1:100, Rabbit, Proteintech, # 12133–2-AP) at 48-h post-transduction with corresponding adenovirus. ..

    Magnetic Beads:

    Article Title: circLRCH3-FUS interaction stabilizes IFI35 mRNA, inhibiting autophagy and aggravating podocyte pyroptosis in lupus nephritis.
    Article Snippet: Background: Lupus nephritis (LN) can severely compromise kidney function, with podocyte injury recognized as a key pathogenic event.. This study aimed to elucidate the role of circular RNA circLRCH3 in LN and to clarify its underlying molecular mechanisms.. Methods: The expression of circLRCH3 was evaluated in renal tissues and serum samples from LN patients, as well as in human podocyte cells (HPCs) treated with LN-derived immunoglobulin G (LN-IgG).

    Article Title: Splicing factor FUS facilitates the progression of PIT1-lineage PitNETs by upregulating MDM2
    Article Snippet: GH3 and MMQ cells were lysed and subjected to RIP with an EZ-Magna kit (17-701, Merck Millipore). .. After overnight incubation at 4 °C with magnetic beads coated with anti-FUS antibody (11570-1-AP, Proteintech), the immunocomplexes were washed 6 times and digested with proteinase K. Total RNA was isolated with phenol/chloroform/isoamyl alcohol (125:24:1), followed by qPCR or sequencing (LC Bio Corporation). ..

    Control:

    Article Title: circLRCH3-FUS interaction stabilizes IFI35 mRNA, inhibiting autophagy and aggravating podocyte pyroptosis in lupus nephritis.
    Article Snippet: Background: Lupus nephritis (LN) can severely compromise kidney function, with podocyte injury recognized as a key pathogenic event.. This study aimed to elucidate the role of circular RNA circLRCH3 in LN and to clarify its underlying molecular mechanisms.. Methods: The expression of circLRCH3 was evaluated in renal tissues and serum samples from LN patients, as well as in human podocyte cells (HPCs) treated with LN-derived immunoglobulin G (LN-IgG).

    Isolation:

    Article Title: Splicing factor FUS facilitates the progression of PIT1-lineage PitNETs by upregulating MDM2
    Article Snippet: GH3 and MMQ cells were lysed and subjected to RIP with an EZ-Magna kit (17-701, Merck Millipore). .. After overnight incubation at 4 °C with magnetic beads coated with anti-FUS antibody (11570-1-AP, Proteintech), the immunocomplexes were washed 6 times and digested with proteinase K. Total RNA was isolated with phenol/chloroform/isoamyl alcohol (125:24:1), followed by qPCR or sequencing (LC Bio Corporation). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Splicing factor FUS facilitates the progression of PIT1-lineage PitNETs by upregulating MDM2
    Article Snippet: GH3 and MMQ cells were lysed and subjected to RIP with an EZ-Magna kit (17-701, Merck Millipore). .. After overnight incubation at 4 °C with magnetic beads coated with anti-FUS antibody (11570-1-AP, Proteintech), the immunocomplexes were washed 6 times and digested with proteinase K. Total RNA was isolated with phenol/chloroform/isoamyl alcohol (125:24:1), followed by qPCR or sequencing (LC Bio Corporation). ..

    Sequencing:

    Article Title: Splicing factor FUS facilitates the progression of PIT1-lineage PitNETs by upregulating MDM2
    Article Snippet: GH3 and MMQ cells were lysed and subjected to RIP with an EZ-Magna kit (17-701, Merck Millipore). .. After overnight incubation at 4 °C with magnetic beads coated with anti-FUS antibody (11570-1-AP, Proteintech), the immunocomplexes were washed 6 times and digested with proteinase K. Total RNA was isolated with phenol/chloroform/isoamyl alcohol (125:24:1), followed by qPCR or sequencing (LC Bio Corporation). ..

    Centrifugation:

    Article Title: The role of JPX in regulating FUS/SLC7A11 signaling pathway mediated ferroptosis in keloid fibroblasts and its potential in scar repair.
    Article Snippet: Background: Keloid scar, a fibrotic disease initiated by aberrant fibroblast proliferation, is influenced by ferroptosis.. This investigation aims to elucidate the mechanism of lncRNA JPX regulating ferroptosis in keloid fibroblasts.. Methods and results: We procured 30 samples of keloid tissue and adjacent normal skin tissues from patients undergoing treatment for keloid scars, subsequently isolating fibroblasts from both the keloid lesions and unaffected portions.



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